G1890

Gelatina from porcine skin

powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

• Número de CAS: 9000-70-8
• Número de EC: 232-554-6
• Número MDL: MFCD00081638
• NACRES: NA.75

Propiedades

• origen biológico: Porcine skin
• Nivel de calidad: 200
• esterilidad: sterile
• tpo: Type A
• Línea del producto: BioReagent
• formulario: powder
• envase: pkg of 1 kg; pkg of 100 g; pkg of 500 g
• technique(s): cell culture | mammalian: suitable; electrophoresis: suitable
• cobertura de la superficie: 100‑200 μg/cm²
• solubilidad: H₂O: soluble 50 mg/mL, clear to hazy, faintly yellow
• enviado en: ambient
• temp. de almacenamiento: room temp

Descripción

Descripción general

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. It comprises majorly glycine, proline and hydroxyproline. Gelatin takes up random coil structure after digestion from the triple helical collagen. It differs from type B bovine gelatin at the N-terminal sequence.

Aplicación

Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent, among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Componentes

La gelatina es una mezcla heterogénea de proteínas hidrosolubles de elevados pesos moleculares promedio, presentes en el colágeno. Las proteínas se extraen hirviendo en agua la piel, los tendones, los ligamentos, los huesos, etc.. La gelatina de tipo A procede de tejido curado con ácido. La B procede de tejido curado con cal.

Precaución

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota de preparación

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: nwg
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)