Merck
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G2500

Sigma-Aldrich

Gelatin from porcine skin

gel strength 300, Type A

Synonym(s):
Folipastatin
CAS Number:
Número de EC:
Número MDL:
NACRES:
NA.61

origen biológico

Porcine skin

Nivel de calidad

200

tpo

Type A

ensayo

70-90% (biuret)

formulario

powder

technique(s)

cell culture | mammalian: suitable

fuerza de gel

~300 g Bloom

solubilidad

H2O: soluble 50 mg/mL

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Descripción general

Gelatin is a hydrocolloid. Bovine or porcine bone and hide are its commercial sources.[1] It is a collagen derivative and comprises protein as a major component with minerals and water.[2]

Aplicación

Gelatin from porcine skin has been used:
  • for coating glass-bottomed plates for culturing A375 cells and in gelatin degradation assay[1]
  • for coating glass slides in the histological studies with brain sections[3]
  • in the synthesis of gelatin methacrylate (GelMA) prepolymer for encapsulating cells on to 3D printed hydrogels[4]
  • to prepare thiol-functionalized gelatin (GelSH) hydrogel for 3D projection bioprinting[5]

Gelatin from porcine skin is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Envase

100, 500 g in poly bottle
1 kg in poly bottle

Acciones bioquímicas o fisiológicas

Gelatin polymer is an alternative biomaterial to collagen for cell and tissue culture.[2] It is a much sorted gelling agent useful as a thickener and food ingredient. Gelatin is also used as a foaming and binding agent and as well as in the pharmaceutical industry.[1] It is biodegradable and biocompatible with biomedical applications especially in biomaterials for cardiac and vascular tissue regeneration.[2]

Componentes

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Precaución

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota de preparación

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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