G8132

β-Glucuronidase from limpets (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB)
• Número de EC: 232-606-8
• Número MDL: MFCD00130629

Properties

• origen biológico: limpet (Patella vulgata)
• Nivel de calidad: 300
• tpo: Type L-II
• formulario: lyophilized powder
• specific activity: 1,000,000-3,000,000 units/g solid
• enviado en: wet ice
• temp. de almacenamiento: −20°C

Description

Aplicación

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine.

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Envase

100000, 250000, 500000, 1000000, 2000000 units in poly bottle

1 mu

Acciones bioquímicas o fisiológicas

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate.

Calidad

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Safety Information

• pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H334
• Consejos de prudencia: P261 - P280 - P342 + P311
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)