G8295

β-Glucuronidase from Escherichia coli

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB)
• Número de EC: 232-606-8
• Número MDL: MFCD00130629

Properties

• recombinante: expressed in E. coli overproducing strain
• Nivel de calidad: 300
• formulario: lyophilized powder
• specific activity: ≥10,000,000 units/g protein (30 min assay)
• mol peso: ~290 kDa
• composición: protein, 40-60% biuret
• temp. de almacenamiento: −20°C

Description

Envase

25000, 500000, 2000000 units in glass bottle

Aplicación

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Calidad

Contains buffer salts and stabilizer.

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Safety Information

• pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H334
• Consejos de prudencia: P261 - P280 - P342 + P311
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)