Merck
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G8898

Sigma-Aldrich

Glycine

suitable for electrophoresis, ≥99%

Synonym(s):
Aminoacetic acid, Aminoethanoic acid, Glycocoll
Linear Formula:
NH2CH2COOH
CAS Number:
Molecular Weight:
75.07
Beilstein:
635782
Número de EC:
Número MDL:
eCl@ss:
32160406
ID de la sustancia en PubChem:

Nivel de calidad

ensayo

≥99%

formulario

powder

technique(s)

electrophoresis: suitable
western blot: suitable

impurezas

≤0.01% Insoluble matter

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60
2.35

mp

240 °C (dec.) (lit.)

solubilidad

H2O: 200 mg/mL, clear, colorless to faintly yellow

trazas de anión

chloride (Cl-): ≤70 ppm

trazas de catión

heavy metals (as Pb): ≤20 ppm

absorción

≤0.15 at 280 at 1 M

SMILES string

NCC(O)=O

InChI

1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

InChI key

DHMQDGOQFOQNFH-UHFFFAOYSA-N

Gene Information

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Glycine suitable for electrophoresis, ≥99%

Sigma-Aldrich

G8898

Glycine

Glycine United States Pharmacopeia (USP) Reference Standard

USP

1295800

Glycine

Glycine BioUltra, for molecular biology, ≥99.0% (NT)

Sigma-Aldrich

50046

Glycine

Glycine ACS reagent, ≥98.5%

Sigma-Aldrich

410225

Glycine

assay

≥99%

assay

-

assay

≥99.0% (NT)

assay

≥98.5%

form

powder

form

-

form

crystalline

form

crystals, powder or granules

technique(s)

electrophoresis: suitable, western blot: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

≤0.01% Insoluble matter

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

H2SO4, passes test (darkened), Hydrolyzable subsances, passes test

color

white to off-white

color

-

color

white

color

-

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

Descripción general

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Aplicación

  • Glycine has been used for the preparation of gelatin composites scaffold.
  • It has been used in the elution buffer to extract protein using HiTrap protein G column.
  • It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Acciones bioquímicas o fisiológicas

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Nota de análisis

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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