Merck

GW21443A

Sigma-Aldrich

Anti-EEA1 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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Sinónimos:
Anti-Early endosome antigen 1

biological source

chicken

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

manufacturer/tradename

Genway 15-288-21443A

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EEA1(8411)

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Este artículo
SAB4300682E3906HPA038158
biological source

chicken

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

mouse, rat, human

species reactivity

human

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: 1:500-1:1000

technique(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK cells, western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of human HeLa cells, western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of mouse NIH3T3 cells

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:200-1:500

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General description

The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid protein. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. EEA1 is a autoantigen associated with subacute cutaneous systemic lupus erythematosus and is a coiled-coil protein localized to early endosomes and cytosol.

Immunogen

Immunogen Sequence: GI # 4503469, sequence 307-417
Recombinant early endosome antigen 1, 162kD

Application

Anti-EEA1 antibody produced in chicken is suitable for immunocytochemistry and western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Early Endosome Antigen 1 (EEA1) controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population. Phosphatidylinositol 3-phosphate (PtdIns(3)P) is essential for the localization and function of EEA1.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Daisuke Tanaka et al.
Molecular genetics and metabolism, 109(1), 112-117 (2013-03-19)
The aim of this study was to investigate the genetic background of familial clustering of diabetes using genome-wide linkage analysis combined with exome sequencing. We recruited a Japanese family with a 3-generation history of diabetes. The family comprised 16 members
J M Gaullier et al.
The Journal of biological chemistry, 275(32), 24595-24600 (2000-05-16)
FYVE zinc finger domains, which are conserved in multiple proteins from yeast to man, interact specifically with the membrane lipid phosphatidylinositol 3-phosphate (PtdIns(3)P). Here we have investigated the structural requirements for the interaction of the FYVE finger of the early
Ashari R Kannangara et al.
EMBO reports, 22(10), e51136-e51136 (2021-08-10)
ATG9A, the only multi-pass transmembrane protein among core ATG proteins, is an essential regulator of autophagy, yet its regulatory mechanisms and network of interactions are poorly understood. Through quantitative BioID proteomics, we identify a network of ATG9A interactions that includes
H Stenmark et al.
The Journal of biological chemistry, 271(39), 24048-24054 (1996-09-27)
EEA1, a 162-kDa autoantigen associated with subacute cutaneous systemic lupus erythematosus, is a coiled-coil protein localized to early endosomes and cytosol. At its C terminus, the protein contains a cysteine-rich motif, which is shared with Vps27, Fab1, and Vac1, yeast
S Selak et al.
Neuroscience, 143(4), 953-964 (2006-11-23)
Early endosome antigen 1 (EEA1), a peripheral membrane protein associated with the cytoplasmic face of early endosomes, controls vesicle fusion during endocytosis, as extensively studied in non-neuronal cells. In neurons, early endosomes are involved in recycling of synaptic vesicles and

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