H0887

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS Number: 7365-45-9
• Número MDL: MFCD00006158
• ID de la sustancia en PubChem: 24895414
• NACRES: NA.75

Properties

• esterilidad: sterile-filtered
• Nivel de calidad: 300
• Línea del producto: BioReagent; BioXtra
• formulario: liquid
• concentración: 1 M
• technique(s): cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• pH: 7.0-7.6
• useful pH range: 6.8-8.2
• temp. de almacenamiento: 2-8°C
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used as a component of:

• Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices
• Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity

Precaución

Concentrations of HEPES over 40mM may be toxic to some cell types.

Nota de preparación

Prepared from HEPES free acid and cell culture grade water.

Safety Information

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable