H0887

Sigma-Aldrich

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Número de CAS:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioReagent
BioXtra

form

liquid

concentration

1 M

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.0-7.6

useful pH range

6.8 - 8.2

pH range

7.0 - 7.6

storage temp.

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:
  • Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices
  • Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity

Caution

Concentrations of HEPES over 40mM may be toxic to some cell types.

Preparation Note

Prepared from HEPES free acid and cell culture grade water.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

The role of the endocrine system in feeding-induced tissue-specific circadian entrainment.
Sato M, et al.
Cell Reports, 8(2), 393-401 (2014)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland Nt
Acta Biomaterialia, 8, 925- 936 (2012)
Kaixi Tang et al.
PLoS computational biology, 14(7), e1006303-e1006303 (2018-07-06)
Focal adhesions are protein complexes that anchor cells to the extracellular matrix. During migration, the growth and disassembly of these structures are spatiotemporally regulated, with new adhesions forming at the leading edge of the cell and mature adhesions disassembling at...
Zuzana Keckesova et al.
Nature, 543(7647), 681-686 (2017-03-23)
Post-mitotic, differentiated cells exhibit a variety of characteristics that contrast with those of actively growing neoplastic cells, such as the expression of cell-cycle inhibitors and differentiation factors. We hypothesized that the gene expression profiles of these differentiated cells could reveal...
Chiao-Chen Chen et al.
Analytical chemistry, 85(7), 3621-3628 (2013-02-21)
Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental...
Artículos
This article discusses attachment-dependent cell growth and cell proliferation investigations using the MultiScreen Assay System from Millipore.
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