Merck
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H3034

Sigma-Aldrich

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-HD3
Número MDL:
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

IgG fraction of antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen 50 kDa

species reactivity

human, rat, mouse

envase

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
immunoprecipitation (IP): 1:2,500 using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:10,000 using nuclear extract from HeLa human epithelioid carcinoma cells
western blot: 1:2,000 using whole extract of PC-12 rat pheochromocytoma cell line

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... HDAC3(8841)
mouse ... Hdac3(15183)
rat ... Hdac3(84578)

Related Categories

Descripción general

Histone deacetylase 3 (HDAC3) is an epigenetic modifier and is expressed in various tissues. It belongs to the class I subfamily of histone deacetylases. The HDAC3 gene is mapped on to human chromosome 5q31.3.

Especificidad

Anti-Histone Deacetylase 3 specifically recognises histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin.

Inmunógeno

synthetic peptide corresponding to amino acids 411-428 of human and chicken HDAC3.

Aplicación

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blotting
  • immunoprecipitation

Acciones bioquímicas o fisiológicas

Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
Histone deacetylase 3 (HDAC3) acts as a transcriptional modulator of inflammatory genes. It deacetylates lysine residues of histones. HDAC3 associates with nuclear receptor co-repressor (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRTs) and activates the complex. This protein participates in interleukin-1 expression and inflammatory responses.

Forma física

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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