H3537

HEPES solution

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

• Synonym(s): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS Number: 7365-45-9
• MDL number: MFCD00006158
• PubChem Substance ID: 329815199
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 200
• sterility: 0.2 μm filtered
• form: liquid
• concentration: 1 M
• technique(s): cell culture | mammalian: suitable
• impurities: Bioburden, tested; DNase, RNase, Protease, Nickase, free; endotoxin, tested
• pH: 5.0-6.0
• useful pH range: 6.8-8.2
• cation traces: Fe: <5 ppm; heavy metals (as Pb): <5 ppm
• suitability: suitable for molecular biology
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable