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H3663

Sigma-Aldrich

Monoclonal Anti-HA antibody produced in mouse

clone HA-7, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
NACRES:
NA.56

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA-7, monoclonal

concentration

~1 mg/mL

application(s)

immunocytochemistry: 1.0-2.0 μg/mL
immunoprecipitation (IP): 0.5-2.0 μg
western blot: 0.25-1 μg/mL

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Hemagglutinin (HA) is a glycoprotein present in the membrane of influenza virus. HA exists as a trimer and each monomer at its membrane tip has receptor-binding site. Glycosylation event of HA occurs in endoplasmic reticulum. The head region with the receptor-binding site of the HA is variable. The mouse monoclonal Anti-HA, Clone HA-7 recognizes the human influenza virus hemagglutinin (HA), known as the HA tag. The product is reactive with HA-tagged fusion proteins expressed at either the amino or the carboxy terminus of the fusion protein.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Monoclonal Anti-HA antibody produced in mouse has been used:
  • in immunoblotting
  • in immunocytochemistry
  • in the immunoprecipitation

Biochem/physiol Actions

Hemagglutinin (HA) binds to acid binding and is essential for the initiation of virus infection. It is the key target for the generation of vaccine. HA is the dominant antigen against which humoral immune response is provoked. Vaccines development involves the generation of antibody specific to the variable head and the conserved stalk region. Both the anti-stalk and anti-head antibodies may elicit protective function in influenza disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide as a preservative.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

The receptor-binding domain of influenza virus hemagglutinin produced in Escherichia coli folds into its native, immunogenic structure
DuBois RM, et al.
Journal of Virology, 85(2), 865-872 (2011)
Ribosomal protein L4 interacts with viral protein VP3 and regulates the replication of infectious bursal disease virus
Chen Y, et al.
Virus Research, 211, 73-78 (2016)
A newly cloned ClC-3 isoform, ClC-3d, as well as ClC-3a mediates Cd2+-sensitive outwardly rectifying anion currents
Okada T, et al.
Cellular Physiology and Biochemistry, 33(3), 539-556 (2014)
Yumei Zheng et al.
Nature communications, 10(1), 3600-3600 (2019-08-11)
Autophagy depends on the E2 enzyme, Atg3, functioning in a conserved E1-E2-E3 trienzyme cascade that catalyzes lipidation of Atg8-family ubiquitin-like proteins (UBLs). Molecular mechanisms underlying Atg8 lipidation remain poorly understood despite association of Atg3, the E1 Atg7, and the composite...
Expanded polyglutamine-containing N-terminal huntingtin fragments are entirely degraded by mammalian proteasomes
Juenemann K, et al.
The Journal of Biological Chemistry, 288(38), 27068-27084 (2013)

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