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H7033
PerfectHyb™ Plus Hybridization Buffer
for Northern and Southern blotting, solution
All Photos(5)
Synonym(s):
southern hybridization buffer, northern hybridization buffer, hyb buffer, hybridization solution, hyb solution
NACRES:
NA.25
Nivel de calidad
200
formulario
solution
temp. de almacenamiento
room temp
Descripción general
Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.
Aplicación
Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.
Características y beneficios
- Fast and easy-to-use
- Up to 20x more sensitive than conventional buffers
- Doesn′t require blocking steps
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
- Compatible with radioactive and non-radioactive probes
- Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes
Otras notas
A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.
Información legal
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
Código de clase de almacenamiento
12 - Non Combustible Liquids
WGK
WGK 2
Punto de inflamabilidad F
Not applicable
Punto de inflamabilidad C
Not applicable
Certificate of Analysis
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Certificate of Origin
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Product Information Sheet
Quotes and Ordering
Protocols
Northern and Southern Blot Protocols & Introduction
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
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