Merck
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H7033

Sigma-Aldrich

PerfectHyb Plus Hybridization Buffer

for Northern and Southern blotting, solution

Synonym(s):
southern hybridization buffer, northern hybridization buffer, hyb buffer, hybridization solution, hyb solution
NACRES:
NA.25

Nivel de calidad

200

formulario

solution

temp. de almacenamiento

room temp

Descripción general

Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

Aplicación

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Características y beneficios

  • Fast and easy-to-use
  • Up to 20x more sensitive than conventional buffers
  • Doesn′t require blocking steps
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays
  • Compatible with radioactive and non-radioactive probes
  • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

Otras notas

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

Información legal

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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