Todas las fotos(3)

H7523

Sigma-Aldrich

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número de CAS:
Peso molecular:
238.30
Beilstein:
883043
Número de EC:
Número MDL:
ID de la sustancia en PubChem:

Nivel de calidad

400

línea de producto

BioXtra

ensayo

≥99.5% (titration)

formulario

powder

impurezas

Insoluble matter, passes filter test

residuo de ign. (900 °C)

≤0.1% (as SO4)

pérdida

≤1.0% loss on drying, 110°C

pH

5.0-6.5 (1 M in H2O)

intervalo de pH útil

6.8 - 8.2

pKa (25 °C)

7.5

solubilidad

H2O: 1 M, clear, colorless

trazas de anión

chloride (Cl-): ≤0.05%

trazas de catión

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorción

≤0.035 at 280 in H2O at 1 M
≤0.045 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

¿Está buscando productos similares? Visit Guía de comparación de productos

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:
  • As a component of homogenisation buffer used in protein sample preparation
  • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
  • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
  • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Envase

50, 250 g in poly bottle

Otras notas

Easily compare specifications for HEPES products with the HEPES specification table.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

Certificado de origen

The inhibitory effect of intracerebroventricularly injected interleukin 1? on testosterone secretion in the rat: role of steroidogenic acute regulatory protein.
Ogilvie KM, et al.
Biology of Reproduction, 60(2), 527-533 (1999)
In vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne CF, et al.
Cardiovascular Research, 49(3), 647-658 (2001)
Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma.
Chen WH, et al.
Biomaterials, 30(29), 5523-5533 (2009)
Hsp60-induced tolerance in the rotifer Brachionus plicatilis exposed to multiple environmental contaminants.
Wheelock CE, et al.
Archives of Environmental Contamination and Toxicology, 36(3), 281-287 (1999)
Increased expression of reticulon 3 in neurons leads to reduced axonal transport of $\beta$ site amyloid precursor protein-cleaving enzyme 1.
Deng M, et al.
The Journal of Biological Chemistry, 288(42), 30236-30245 (2013)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico