H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• Número de EC: 230-907-9
• Número MDL: MFCD00006158
• ID de la sustancia en PubChem: 24895796

Properties

• Línea del producto: BioXtra
• Nivel de calidad: 400
• ensayo: ≥99.5% (titration)
• formulario: powder
• impurezas: Insoluble matter, passes filter test
• residuo de ign. (900 °C): ≤0.1% (as SO₄)
• pérdida: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubilidad: H₂O: 1 M, clear, colorless
• trazas de anión: chloride (Cl^-): ≤0.05%
• trazas de catión: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• absorción: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Otras notas

Easily compare specifications for HEPES products with the HEPES specification table.

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)