H9658

Sigma-Aldrich

Monoclonal Anti-HA antibody produced in mouse

clone HA-7, ascites fluid

Sinónimos:
Monoclonal Anti-HA, Sigma Ha Antibody, Ha Antibody Sigma, Anti-HA, HA Antibody - Monoclonal Anti-HA antibody produced in mouse, Anti-Influenza Hemagglutinin
MDL number:

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HA-7, monoclonal

contains

15 mM sodium azide

application(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Monoclonal Anti-HA antibody produced in mouse has been used in:
  • Immunoblotting, to study the expression of fusion protein
  • Coimmunoprecipitation assay
  • Immunoblotting of lysate protein
Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:
  • immunoprecipitation in human embryonic kidney 293 cells
  • immunoblotting in HeLa cells
  • immunopurification in human embryonic kidney 293 cells
  • immunostaining for electron microscopy in mouse embryonic fibroblasts
  • in immunocytochemistry of hippocampal slices

Biochem/physiol Actions

Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.
Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

  6. What is the recommended dilution of Product H9658 - HA antibody for immunoprecipitation and for indirect immunostaining?

    H9658 - Monoclonal Anti-HA antibody produced in mouse is the ascites fluid. It is not purified. We only test this product in western blotting.  This product contains 15-55 mg/ml total protein with 4-7 mg/ml specific antibody. H3663 - Monoclonal Anti-HA antibody produced in mouse as the purified immunoglobulin. We test this product for immunoprecipitation using 0.5-2.0 μg and for immunocytochemistry using 1.0-2.0 μg/mL.These ranges would be a good starting concentration. In order to obtain best results in different techniques and preparations, we recommend determining optimal working dilution by titration test.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Receptor binding and pH stability?how influenza A virus hemagglutinin affects host-specific virus infection
Mair CM, et al.
Biochimica et Biophysica Acta - Biomembranes, 1838(4), 1153-1168 (2014)
The role of influenza A virus hemagglutinin residues 226 and 228 in receptor specificity and host range restriction.
Vines A, et al.
Journal of Virology, 72(9), 7626-7631 (1998)
Analysis of influenza virus hemagglutinin receptor binding mutants with limited receptor recognition properties and conditional replication characteristics
Bradley KC, et al.
Journal of Virology, 85(23), 12387-12398 (2011)
Synaptic targeting and functional modulation of GluK1 kainate receptors by the auxiliary neuropilin and tolloid-like (NETO) proteins.
Copits B A, et al.
The Journal of Neuroscience, 31(20), 7334-7340 (2011)
Functional mutations in mouse norepinephrine transporter reduce sensitivity to cocaine inhibition.
Wei H, et al.
Neuropharmacology, 56(2), 399-404 (2009)

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