HT5011

Sigma-Aldrich

Formalin solution, neutral buffered, 10%

case of 48 × 15 mL, histological tissue fixative

MDL number:
PubChem Substance ID:
NACRES:
NA.47

shelf life

Expiry date on the label.

packaging

case of 48 × 15 mL

concentration

10% formalin (approx. 4% formaldehyde)

application(s)

hematology: suitable
histology: suitable

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

storage temp.

room temp

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.

Application

10% neutral buffered formalin (NBF) is a popular general purpose histological fixative. It has been used to fix tissues, protein complexes and tumor samples.
Popular general purpose histological fixative.

Packaging

Case containers are polypropylene with white leak-proof lids and are partially prefilled with 10% NBF.

Physical form

Neutral buffered solution

Storage and Stability

Store tightly closed at room temperature. Do not freeze.

pictograms

Exclamation markHealth hazard

signalword

Danger

hazcat

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Flash Point F

185.0 °F

Flash Point C

85 °C

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

Biological characterization of a Stable Effector Functionless (SEFL) monoclonal antibody scaffold in vitro.
Liu L, et al.
The Journal of Biological Chemistry, 292(5), 1876-1883 (2017)
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Frontiers in cellular and infection microbiology, 8, 16-16 (2018-02-17)
Most bacterial infections initiate at the mucosal epithelium lining the gastrointestinal, respiratory, and urogenital tracts. At these sites, bacterial pathogens must adhere and increase in numbers to effectively breach the outer barrier and invade the host. If the bacterium succeeds...
Nuclear GSK3β induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastoma.
Yang Y, et al.
International Journal of Oncology (2018)
Mangiferin induces islet regeneration in aged mice through regulating p16INK4a.
Wang H, et al.
International Journal of Molecular Medicine, 41(6), 3231-3242 (2018)
Benedicte Stavik et al.
Journal of hematology & oncology, 6, 5-5 (2013-01-17)
Tissue factor (TF) pathway inhibitor-1 (TFPI) is expressed in several malignant tissues- and cell lines and we recently reported that it possesses anti-tumor effects in breast cancer cells, indicating a biological role of TFPI in cancer. The two main splice...

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