The accepted molar extinction coefficient for human IgG is ~210,000 cm-1M-1. The A0.1%280 value is 1.38.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdf
I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sinónimos:
IgG humana
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 10 mg | Comprobar disponibilidad del carrito | MXP 1,931.00 | |
| 50 mg | Disponible para enviar HOYdesdeToluca | MXP 6,944.00 | |
| 100 mg | Comprobar disponibilidad del carrito | MXP 10,248.00 | |
| 500 mg | Comprobar disponibilidad del carrito | MXP 39,217.00 |
Acerca de este artículo
Conjugate:
unconjugated
Technique(s):
ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable
Application:
DIBA, ELISA, IEP, WB
Citations:
228
Servicio técnico
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Permítanos ayudarleconjugate
unconjugated
Quality Level
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable
storage temp.
2-8°C
General description
La IgG humana purificada se aísla de suero combinado humano sano por fraccionamiento y cromatografía de intercambio iónico. El producto se suministra como un polvo liofilizado esencialmente exento de sal (menos del 1 % de sodio).
Application
La IgG del suero humano se utilizó como patrón a una concentración de 1274 mg/ml en ELISA para determinar las respuestas de anticuerpos a Porphyromonas gingivalis y a Prevotella intermedia.[1] Se utilizó para el inmunomarcaje de las células Vero, BHK y PtK2 a una concentración de 0,2 mg/ml.[2] La IgG del suero humano se utilizó como patrón en la SDS-PAGE de anticuerpos contra el LPS de Shigella.[3]
Biochem/physiol Actions
El subtipo de anticuerpo IgG es el más abundante de las inmunoglobulinas séricas del sistema inmunitario. Las IgG son secretadas por las células B y se encuentran en la sangre y los líquidos extracelulares; proporcionan protección frente a infecciones causadas por bacterias, hongos y virus. Las IgG maternas son transferidas al feto a través de la placenta, que es vital para la defensa inmunitaria del neonato frente a las infecciones.[4]
Disclaimer
Salvo que se especifique lo contrario en nuestro catálogo o en otra documentación de la empresa que acompañe al producto, nuestros productos están indicados sólo para investigación y no deben utilizarse para ningún otro propósito, como, entre otros, usos comerciales no autorizados, diagnóstico in vitro, usos terapéuticos ex vivo o in vivo o cualquier tipo de consumo o aplicación en humanos o animales.
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Este artículo | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| technique(s) ELISA: suitable, immunoelectrophoresis: suitable, dot immunobinding: suitable, western blot: suitable | technique(s) - | technique(s) - | technique(s) - |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| composition Protein, ≥90% | composition - | composition - | composition - |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Datasheet
Nils A Brechmann et al.
Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
Youhei Egami et al.
Journal of cell science, 130(24), 4168-4179 (2017-11-09)
Phagosome formation is a complicated process that requires spatiotemporally regulated actin reorganization. We found that RhoC GTPase is a critical regulator of FcγR-mediated phagocytosis in macrophages. Our live-cell imaging revealed that RhoC, but not RhoA, is recruited to phagocytic cups
Nak-Hyeon Kim et al.
Sensors (Basel, Switzerland), 19(8) (2019-04-24)
Surface plasmon resonance (SPR) sensors based on a silver film suffer from signal degradation due to silver oxidation in aqueous sensing environments. To overcome this limitation, we fabricated the planar plasmonic substrate employing an atomic MoS2 layer on a silver
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| I4506-500MG | 04061833856864 |
| I4506-100MG | 04061833856840 |
| I4506-50MG | 04061833856871 |
| I4506-10MG | 04061833856857 |
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Hello, I was wondering if the molar extinction coefficient is available for the reference i4506-500mg? Thanks for your help
1 answer-
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１．本IgG試薬は、何人の健常人の血漿プールから得られたものでしょうか？ ２．本IgG試薬は医薬品「静注用免疫グロブリン（IVIG）」と同等品と考えてよろしいでしょうか？ 炎症抑制活性が確認された例はあるでしょうか？ 精製度はIVIGと比較してどうでしょうか？
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The number of donors is not recorded for the original starting raw material. Historically, donor numbers for the plasma raw material are estimated to range from 1600 to 2000 individuals. No anti-inflammatory characterization has been performed. This product is intended for research use only. Unless otherwise stated this product is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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What is the proportion of IgG1, IgG2, IgG3 and IgG4 in this product?
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In human serum, the 4 subclasses of human IgG are present at the approximate proportions of 66%, 23%, 7% and 4%, respectively.
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How is this standard reconstituted?
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The product is essentially a salt-free preparation and should be reconstituted to a minimum concentration of 1 mg/ml with a saline solution containing 150 mM NaCl.
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What is the expected MW of the product?
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The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
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The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
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What is the isoelectric point of this
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This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
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As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdfHelpful?
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
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After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
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The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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