I8260

Sigma-Aldrich

IgM from human serum

reagent grade, ~95% (HPLC), buffered aqueous solution

Sinónimos:
Human IgM
MDL number:

Quality Level

grade

reagent grade

assay

~95% (HPLC)

form

buffered aqueous solution

conjugate

unconjugated

shipped in

wet ice

storage temp.

2-8°C

General description

There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.

Application

Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml) and dot blot assay. Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays.
IgM from human serum has been used
  • in ELISA inhibition assay
  • in immunoblotting
  • as MW (molecular weight) standards
  • in IgM binding assay
  • to confirm the depletion of immunoglobulins

Packaging

10 mg in serum bottle
1, 5 mg in glass bottle

Biochem/physiol Actions

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.

Physical form

Solution in 0.05 M Tris-HCl, 0.2 M sodium chloride, pH 8.0, containing 15 mM sodium azide.

Storage and Stability

Store at 2-8 °C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

nwg

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

L Rydberg et al.
Xenotransplantation, 5(2), 105-110 (1998-12-16)
A quantitative ELISA technique for determination of human anti-pig xenoantibody number in serum samples has been established using pig lymphocytes and pig/rabbit erythrocytes as target cells and a pool of serum from human blood group AB donors. The number of...
Apolline Salama et al.
Transplantation, 101(10), 2501-2507 (2017-02-16)
Polyclonal antihuman thymocyte rabbit IgGs (antithymocyte globulin [ATG]) are popular immunosuppressive drugs used to prevent or treat organ or bone-marrow allograft rejection, graft versus host disease, and autoimmune diseases. However, animal-derived glycoproteins are also strongly immunogenic and rabbit ATG induces...
Isabelle Leduc et al.
Infection and immunity, 77(2), 657-666 (2008-11-19)
Resisting the bactericidal activity of naturally occurring antibodies and complement of normal human serum is an important element in the evasion of innate immunity by bacteria. In the gram-negative mucosal pathogen Haemophilus ducreyi, serum resistance is mediated primarily by the...
Jonathan Hoare et al.
The journal of gene medicine, 7(3), 325-333 (2004-11-03)
The baculovirus Autographa californica nucleo-polyhedrosis virus (AcNPV) is an alternative to other viral vectors for hepatic gene delivery. A barrier to AcNPV being used in vivo is its susceptibility to inactivation by serum complement 1. In vivo utility has only...
H R Amini et al.
FEMS immunology and medical microbiology, 16(3-4), 163-172 (1996-12-31)
Western blot analysis (immunoblotting) of cell surface-associated proteins from Helicobacter pylori confirmed our previous findings that binding of human IgG is a common property (among H. pylori strains). Purification of the IgG-binding proteins (IGBP) was achieved by two purification steps...

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico