Merck

I9018

Sigma-Aldrich

Monoclonal Anti-Involucrin antibody produced in mouse

clone SY5, ascites fluid

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Sinónimos:
Anti-IVL
Número MDL:

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

SY5, monoclonal

contiene

15 mM sodium azide

species reactivity

canine, gorilla, owl monkey, pig, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

Gene Information

human ... IVL(3713)

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Este artículo
SAB4200794SAB4501594V2258
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

ascites fluid

clone

SY5, monoclonal

clone

SY5, monoclonal

clone

polyclonal

clone

LN-6, monoclonal

species reactivity

canine, gorilla, owl monkey, pig, human

species reactivity

canine, gorilla, porcine, mouse, human, owl monkey

species reactivity

human

species reactivity

pig, mouse, sheep, human, bovine, rabbit, feline, rat

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections, western blot: suitable

technique(s)

immunoblotting: suitable, immunofluorescence: suitable, immunohistochemistry: 2.5-5 μg/mL using human skin sections, immunoprecipitation (IP): suitable

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections, immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable using cultured cells, western blot: suitable

Descripción general

Involucrin is a soluble precursor protein that regulates terminal differentiation in keratinocytes. This protein is initially present in the cytosol component of keratinocytes and later associates with membrane proteins. Involucrin is expressed in squamous cell cancer and its expression is down-regulated by HPV 16 oncoproteins . Monoclonal Anti-Involucrin is specific for involucrin in primate and non-primate mammals. In cultured human keratinocytes, the antibody stains a 120 kDa band by immunoblot under denaturing-reducing conditions. However, in other cells the antibody binds to involucrin protein in varied sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approx. 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet at approx 105 kDa in pig. The antibody does not bind to mouse epidermis; hence the antibody can be used to study human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies .

Especificidad

The antibody detects involucrin in a range of primate and nonprimate mammals in a variety of techniques. In immunoblotting of cultured human keratinocytes, the antibody stains a 120 kDa band under denaturing and reducing conditions. However, in other systems, the antibody stains the involucrin protein in a variety of sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approximately 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of ~105 kDa in pig. The antibody does not react with mouse epidermis, permitting use in studies of human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies.

Inmunógeno

human involucrin.

Aplicación

Monoclonal Anti-Involucrin antibody is suitable for use in immunofluorescence at 1:100 dilutions using keratinocytes or for indirect immunofluorescence using methanol fixed mouse embryonic fibroblasts-keratinocytes co-cultures . The antibody may also be used for western blot (1:1000) and immunohistochemical analysis . Additionally, the product can be useful for indirect ELISA, immunoblotting, immunoprecipitation and immunocytochemistry.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Joan Manils et al.
Oncotarget, 6(26), 22375-22396 (2015-06-20)
TREX2 is a 3'-DNA exonuclease specifically expressed in keratinocytes. Here, we investigated the relevance and mechanisms of TREX2 in ultraviolet (UV)-induced skin carcinogenesis. TREX2 expression was up-regulated by chronic UV exposure whereas it was de-regulated or lost in human squamous
D L Hudson et al.
Hybridoma, 11(3), 367-379 (1992-06-01)
Involucrin is a precursor of the insoluble protein envelope that is assembled in the outermost layers of the epidermis. The coding sequence of the protein contains a number of short tandem repeats that have been greatly altered during mammalian evolution.
Jun Dai et al.
PloS one, 8(7), e70392-e70392 (2013-08-08)
RORα is a retinoid-related orphan nuclear receptor that regulates inflammation, lipid metabolism, and cellular differentiation of several non-epithelial tissues. In spite of its high expression in skin epithelium, its functions in this tissue remain unclear. Using gain- and loss-of-function approaches
M N Ruesch et al.
Journal of virology, 72(6), 5016-5024 (1998-05-30)
The life cycle of the papillomaviruses is closely linked to host cell differentiation, as demonstrated by the fact that amplification of viral DNA and transcription of late genes occur only in the suprabasal cells of a differentiated epithelium. Previous studies
Z Suo et al.
Histopathology, 23(1), 45-54 (1993-07-01)
The expression of cytokeratins (CK) 1, 4, 5/6, 8, 13, 18, 19 and 20 and involucrin in 42 cases of squamous cell carcinomas from various locations was examined. The tumours expressed CK5/6 in 55%, CK8 in 76%, CK13 in 43%

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