I9516

2-Propanol

BioReagent, for molecular biology, ≥99.5%

• Synonym(s): sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
• Linear Formula: (CH₃)₂CHOH
• CAS Number: 67-63-0
• Molecular Weight: 60.10
• Beilstein: 635639
• Número de EC: 200-661-7
• Número MDL: MFCD00011674
• ID de la sustancia en PubChem: 24896158
• NACRES: NA.52

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• densidad de vapor: 2.1 (vs air)
• presión de vapor: 33 mmHg ( 20 °C); 44 mmHg ( 25 °C)
• Línea del producto: BioReagent
• ensayo: ≥99.5%
• formulario: liquid
• temp. de autoignición: 750 °F
• lim. expl.: 2.0-12.7 %, 93 °C
• technique(s): DNA extraction: suitable; RNA extraction: suitable
• impurezas: ≤0.2% water; water
• color: colorless
• índice de refracción: n20/D 1.377 (lit.)
• bp: 82 °C (lit.)
• mp: −89.5 °C (lit.)
• solubilidad: water: soluble (completely)
• densidad: 0.785 g/mL at 25 °C (lit.)
• idoneidad: suitable for precipitation of nucleic acids
• temp. de almacenamiento: room temp
• SMILES string: CC(C)O
• InChI: 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
• InChI key: KFZMGEQAYNKOFK-UHFFFAOYSA-N

Description

Descripción general

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

• DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
• Protein crystallization.
• Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Aplicación

2-Propanol has been used:

• in DNA and RNA extraction
• for determination of hydroxyproline levels(The Merck Index, 12th ed)
• for checking cell viability by MTT assay
• in microarray hybridization

Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

Envase

25, 25, 500 mL in glass bottle

1, 4 L

Safety Information

• pictogramas: GHS02,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H225 - H319 - H336
• Consejos de prudencia: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 3 - Flammable liquids
• WGK: WGK 1
• Punto de inflamabilidad F: 53.6 °F - closed cup
• Punto de inflamabilidad C: 12.0 °C - closed cup
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter