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BioReagent, for molecular biology, ≥99.5%

IPA, Isopropanol, sec-Propyl alcohol, Isopropyl alcohol
Fórmula lineal:
Número de CAS:
Peso molecular:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


for molecular biology

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line






autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C


DNA extraction: suitable
RNA extraction: suitable


≤0.2% water



refractive index

n20/D 1.377 (lit.)


82 °C (lit.)


−89.5 °C (lit.)


water: soluble (completely)


0.785 g/mL at 25 °C (lit.)


suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string




InChI key


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General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
  • Protein crystallization.
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.


2-Propanol has been used:
  • in DNA and RNA extraction
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.


25, 500 mL in glass bottle


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Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK Germany


Flash Point F

53.6 °F - closed cup

Flash Point C

12.0 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificado de Análisis

Certificado de origen

Robert B Nicewonger et al.
Bioorganic & medicinal chemistry letters, 12(14), 1799-1802 (2002-06-28)
In this paper, we describe the synthesis of N-(6-cyano-1,3-dimethyl-2,4-dioxo-5-substituted-1,3-dihydropyridino[2,3-d] pyrimidin-7-yl)imides 1. We will show the synthesis of 1 using both conventional heating and microwave techniques. In addition, the imide was attached to polystyrene and this immobilized imide was equally as...
S Knapp et al.
Proteins, 23(4), 595-597 (1995-12-01)
The thermostable amylopullulanase from Pyrococcus woesei was crystallized. Crystals, suitable for a crystallographic analysis up to a size of 0.6 mm in their longest dimension, have been obtained by the vapor diffusion method in a solution containing polyethyleneglycol 4000 (PEG...
Peter K Davidsen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 110(2), 309-317 (2010-10-30)
MicroRNAs (miRNA), small noncoding RNA molecules, may regulate protein synthesis, while resistance exercise training (RT) is an efficient strategy for stimulating muscle protein synthesis in vivo. However, RT increases muscle mass, with a very wide range of effectiveness in humans....
M J Romão et al.
European journal of biochemistry, 215(3), 729-732 (1993-08-01)
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as...
Christudas Morais et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(5), 1462-1474 (2009-12-29)
Renal cell carcinoma (RCC) is a highly metastatic and lethal disease with few efficacious treatments. Many studies have shown that the ubiquitous transcription factor nuclear factor kappa B (NF-kappaB) plays a key role in the development and progression of many...


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Isopropanol Molecular Biology Reagent

Isopropanol is a polar organic solvent that is widely used in chemistry, biochemistry and molecular biology

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