L2020

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

• Synonym(s): Laminin
• CAS Number: 114956-81-9
• Número MDL: MFCD00081739
• NACRES: NA.75

Properties

• origen biológico: mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
• Nivel de calidad: 300
• Línea del producto: BioReagent
• formulario: aqueous solution
• mol peso: A subunit 400 kDa; B1 subunit 210 kDa; B2 subunit 200 kDa
• envase: pkg of 1 mg
• concentración: 1-2 mg/mL in Tris-buffered saline
• technique(s): cell culture | mammalian: suitable
• cobertura de la superficie: 1‑2 μg/cm²
• Nº de acceso UniProt: P02468; P02469; P19137; Q61092
• enviado en: dry ice
• temp. de almacenamiento: −20°C
• Gene Information: mouse ... Lama1(16772) , Lamb1(16777) , Lamc1(226519) , Lamc2(16782)

Description

Aplicación

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:

• insulin-producing cell (IPC) differentiation.
• Infection inhibition assays.
• Proliferation Assay.

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm². The optimal concentration does depend on cell type as well as the application or research objectives.

Acciones bioquímicas o fisiológicas

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Componentes

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Precaución

It is recommended to store this product in working aliquots at -20°C.

Nota de preparación

This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Safety Information

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)