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L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

Sinónimos:
Luria Broth Miller, Luria-Bertani broth, Miller
NACRES:
NA.85

grado

for molecular biology

Nivel de calidad

esterilidad

sterile; 0.2 μm filtered

formulario

liquid

pH

6.5-7.2

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

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Este artículo
L3522L3147L2897
LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

liquid

form

powder

form

powder

form

-

pH

6.5-7.2

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2(4% solution)

pH

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

Descripción general

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Aplicación

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Características y beneficios

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Componentes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Las referencias de los producto se combinan con los tamaños de envase y las cantidades cuando se muestran en la página web, por ejemplo: T1503-25G. Asegúrese de introducir el número de producto ÚNICAMENTE en el campo del Número de producto (Ejemplo T1503).

Ejemplo

T1503
Referencia del producto
-
25G
Tamaño del envase/cantidad

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Artículos

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protocolos

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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