L3147

LB Broth with agar (Miller)

Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

• Sinónimos: Luria Agar, Luria Bertani agar, Miller’s LB agar, lysogeny broth agar
• eCl@ss: 42040102
• NACRES: NA.85

Propiedades

• grado: for molecular biology
• esterilidad: non-sterile
• formulario: powder
• composición: Agar, 15 g/L ; NaCl, 10 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• pH: 6.8-7.2(4% solution)
• application(s): food and beverages
• temp. de almacenamiento: room temp
• idoneidad: nonselective for Escherichia coli; nonselective for coliforms

Descripción

Descripción general

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Aplicación

LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440, plating E. coli and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Características y beneficios

Miller′s LB powder with agar provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to pre-poured plates
• Standard formulation

Nota de preparación

1. Suspend 40 g in 1L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitución

Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable