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Merck

L3522

Caldo LB (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Sinónimos:

Caldo LB de Miller, Caldo Luria

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250 G

MXP 1,995.00

1 KG

MXP 6,034.00

MXP 1,995.00


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NACRES:
NA.85
UNSPSC Code:
41106200

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grade

Molecular Biology

description

Miller′s Modification, 10 g/L NaCl

sterility

non-sterile

form

powder

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

pH

6.8-7.2(2.5% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Quality Level

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1 of 4

Este artículo
L2542L3147L3022
suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

form

powder

form

liquid

form

powder

form

powder

composition

NaCl, 10 g/L , Yeast Extract, 5 g/L , Tryptone, 10 g/L

composition

-

composition

Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

application(s)

food and beverages

application(s)

microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

pH

6.8-7.2(2.5% solution)

pH

6.5-7.2

pH

6.8-7.2(4% solution)

pH

6.8-7.2(2% solution)

General description

El caldo Luria de Miller es un medio para crecimiento microbiano muy citado que se utiliza para el cultivo de E. coli. Este caldo microbiano rico en nutrientes contiene péptidos, aminoácidos, vitaminas hidrosolubles y carbohidratos.

Application

El caldo LB (Miller) se ha utilizado como medio de cultivo para E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus y muestras de suelo para el aislamiento del género Bacillus .
Idóneo para cultivo no selectivo de cepas de E. coli para clonación, producción de plásmidos ADN y producción de proteínas recombinantes. También es adecuado para el cultivo selectivo cuando se añaden los antibióticos apropiados.

Biochem/physiol Actions

El caldo Luria se utiliza para el mantenimiento y la propagación de Escherichia coli. E. coli crece más deprisa en el caldo Luria porque la triptona y la levadura proporcionan factores de crecimiento esenciales que, de otro modo, E. coli tendría que sintetizar. El caldo Luria contiene también electrólitos esenciales para el transporte y el equilibrio osmótico, debido al componente NaCl.

Features and Benefits

El polvo de LB de Miller proporciona:
  • Fácil escalado utilizando tamaños de envase mayores
  • Una alternativa económica al líquido
  • Una formulación estándar

Preparation Note

1. Suspenda 25 g en 1 l de agua destilada.
2. Esterilice en autoclave durante 15 minutos a 121 ºC.
Para preparar el medio de Luria, Adams y Ting (también conocido como caldo LC: Agregue asépticamente 25 ml de cloruro de calcio 0,1 M estéril después de haberlo esterilizado en autoclave.
Agite hasta suspender 25 g de polvo en 1 l de agua. Esteriliza en autoclave durante 15 minutos a 121 ºC. Deje enfriar antes de añadir más compuestos, como los antibióticos (si se desea).

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Selecting lipopeptide-producing, Fusarium-suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10. 5
Adeniji A, et al.
MicrobiologyOpen, e742-e742 (2018)
Demeng Tan et al.
Microorganisms, 8(3) (2020-03-12)
Phage therapy is a potential and promising avenue for controlling the emergence and spread of multidrug-resistant (MDR) Klebsiella pneumoniae, however, the rapid development of anti-phage resistance has been identified as an obstacle to the development of phage therapy. Little is
Single-cell bacteria growth monitoring by automated DEP-facilitated image analysis
Peitz I and van Leeuwen R
Lab on a chip, 10(21), 2944-2951 (2010)
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
Transcriptional profiling of Vibrio parahaemolyticus exsA reveals a complex activation network for type III secretion
Liu AC and Thomas NA
Frontiers in Microbiology, 6, 1089-1089 (2015)

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Questions

1–6 of 6 Questions  
  1. What is the recommended duration for using this item after autoclaving, and can it be stored at 4 degrees Celsius for extended or long-term use?

    1 answer
    1. The product is stable for nearly 3 years when stored at room temperature. There is no specific requirement to store L2542 at 2-8°C. However, if the container is opened and closed, there is a possibility of contamination through routine use.

      Helpful?

  2. Will adding magnesium to the culture medium increase cell density?

    1 answer
    1. In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator.

      Helpful?

  3. What is the difference among the LB - Miller products?

    1 answer
    1. LB - Miller is available in many types to suit your needs.  The different product formats include powder and liquid form.  The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)

      Helpful?

  4. What are the differences among the Luria, Lennox and Miller LB formulations?

    1 answer
    1. LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version).

      Helpful?

  5. Which bacterial culture medium is the best choice for my application?

    1 answer
    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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