Merck

L7658

Sigma-Aldrich

LB Broth (Lennox)

EZMix powder microbial growth medium

Sinónimos:
Lennox broth
NACRES:
NA.85

grado

for molecular biology

Nivel de calidad

descripción

quick-dissolve, dust-free formulation

esterilidad

non-sterile

formulario

powder

pH

6.8-7.2(2.5% solution)

application(s)

agriculture
food and beverages

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

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Este artículo
L3022L3522L7275
LB Broth (Lennox) EZMix™ powder microbial growth medium

Sigma-Aldrich

L7658

LB Broth (Lennox)

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

LB Broth (Lennox)

Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

LB Broth (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7275

LB Broth (Lennox)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

tablet

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2(2% solution)

pH

6.8-7.2(2.5% solution)

pH

-

application(s)

agriculture
food and beverages

application(s)

food and beverages

application(s)

food and beverages

application(s)

food and beverages

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Descripción general

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.

Aplicación

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Características y beneficios

Lennox LB EZMix powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Componentes

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)

Nota de preparación

1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.

Reconstitución

Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Información legal

EZMix is a trademark of Merck KGaA, Darmstadt, Germany
Zeocin is a registered trademark of Cayla Sarl

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Las referencias de los producto se combinan con los tamaños de envase y las cantidades cuando se muestran en la página web, por ejemplo: T1503-25G. Asegúrese de introducir el número de producto ÚNICAMENTE en el campo del Número de producto (Ejemplo T1503).

Ejemplo

T1503
Referencia del producto
-
25G
Tamaño del envase/cantidad

Otros ejemplos

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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  • Si encuentra un número de lote como TO09019TO, introduzca el número de lote 09019TO sin las dos primeras letras.

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Katherine A Kantardjieff et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 5), 895-902 (2004-04-23)
The Mycobacterium tuberculosis rmlC gene encodes dTDP-4-keto-6-deoxyglucose epimerase, the third enzyme in the M. tuberculosis dTDP-L-rhamnose pathway which is essential for mycobacterial cell-wall synthesis. Because it is structurally unique, highly substrate-specific and does not require a cofactor, RmlC is considered
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The first in vivo measurements of a protein diffusion coefficient versus cytoplasmic biopolymer volume fraction are presented. Fluorescence recovery after photobleaching yields the effective diffusion coefficient on a 1-mum-length scale of green fluorescent protein within the cytoplasm of Escherichia coli
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Protocolos

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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