Merck
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L8629

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Erythroagglutinin PHA-E

Synonym(s):
PHA, Phaseolus vulgaris agglutinin
NACRES:
NA.32

Nivel de calidad

300

formulario

salt-free, lyophilized powder

potencia

<10 μg per mL agglutination activity (with erythryocytes)

composición

Protein, ≥80% biuret

temp. de almacenamiento

2-8°C

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Envase

5 mg in glass bottle

Acciones bioquímicas o fisiológicas

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Calidad

Tested for leucoagglutination.

Nota de preparación

Purified by a modification of the method of Leavitt.

Nota de análisis

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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