LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• Synonym(s): API, Lysyl endopeptidase, Protease I
• Enzyme Commission number: 3.4.21.50 (BRENDA, IUBMB)

Properties

• origen biológico: Achromobacter lyticus
• Nivel de calidad: 200
• recombinante: expressed in E. coli (206-473aa)
• grado: Proteomics Grade
• formulario: ready-to-use solution
• concentración: 1 mg/mL
• pH óptimo: 8.5
• pH range: 9.0-9.5(catalytic)
• Nº de acceso UniProt: P15636
• enviado en: dry ice
• temp. de almacenamiento: −20°C

Description

Descripción general

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Especificidad

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Aplicación

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Envase

1mg/ml in Plastic

Nota de preparación

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Almacenamiento y estabilidad

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Otras notas

For R&D only.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable