M0699

MSK1, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MGC1911, MSPK1, RLPK, RPS6KA5
• NACRES: NA.32

Properties

• recombinante: expressed in baculovirus infected Sf9 cells
• Nivel de calidad: 200
• Línea del producto: PRECISIO^® Kinase
• ensayo: ≥70% (SDS-PAGE)
• formulario: buffered aqueous glycerol solution
• specific activity: 75-103 nmol/min·mg
• mol peso: ~120 kDa
• Nº de acceso UniProt: O75582
• enviado en: dry ice
• temp. de almacenamiento: −70°C
• Gene Information: human ... RPS6KA5(9252)

Description

Acciones bioquímicas o fisiológicas

MSK1 or mitogen- and stress-activated protein kinase-1 contains 2 protein kinase domains and shares 43% protein sequence identity with the MAPKAPK1 isoforms. Northern blot analysis shows that MSK1 is expressed in all tissues with the highest level of expression in brain, muscle, and placenta. Immunoelectron microscopy localized MSK1 to the nucleus. MSK1 is activated in vitro and in vivo by either ERK or SAPK2 proteins. MSK1 rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals. By radiation hybrid analysis, the RPS6KA5 gene has been mapped to chromosome 14q31-q32.

Forma física

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Información legal

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable