Merck

M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

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Sinónimos:
MgCl2, dichloromangesium, magnesium chloride
Fórmula lineal:
MgCl2
Número de CAS:
Peso molecular:
95.21
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.52

grado

for molecular biology

Nivel de calidad

esterilidad

non-sterile; 0.2 μm filtered

formulario

liquid

concentración

1.00 M±0.01 M

impurezas

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

solubilidad

water: soluble

densidad

1.080 g/cm3

trazas de catión

heavy metals (as Pb): ≤5 ppm

actividad extraña

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Este artículo
684750045763036
Magnesium chloride solution BioUltra, for molecular biology, 2 M in H2O

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68475

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Magnesium chloride solution BioUltra, for molecular biology, ~0.025 M in H2O

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form

liquid

form

liquid

form

-

form

-

impurities

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total nitrogen (N)

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total nitrogen (N)

solubility

water: soluble

solubility

-

solubility

-

solubility

-

density

1.080 g/cm3

density

-

density

1.01 g/mL at 20 °C

density

-

cation traces

heavy metals (as Pb): ≤5 ppm

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤10 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤500 mg/kg, Li: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤100 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

-

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤10 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤500 mg/kg, Li: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤1000 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

Descripción general

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Aplicación

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Características y beneficios

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

Componentes

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


Certificates of Analysis (COA)

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Las referencias de los producto se combinan con los tamaños de envase y las cantidades cuando se muestran en la página web, por ejemplo: T1503-25G. Asegúrese de introducir el número de producto ÚNICAMENTE en el campo del Número de producto (ejemplo: T1503).

Ejemplo

T1503
Referencia del producto
-
25G
Tamaño del envase/cantidad

Otros ejemplos

705578-5MG-PW

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Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria, 34(2), 149-157 (2019)
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Protocolos

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

Enzymatic Assay of Hexokinase

To measure hexokinase activity, a continuous spectrophotometric rate-determination assay is used at 340 nm. Hexokinase catalyzes the phosphorylation of D-hexose sugars using ATP as the phosphate source.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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