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Merck

M1028

Magnesium chloride solution

Molecular Biology, 1.00 M±0.01 M

Magnesium chloride solution

Sinónimos:

MgCl2, dichloromagnesium, magnesium chloride

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10 X 1 ML

MXP 1,699.00

100 ML

MXP 1,963.00

MXP 1,699.00


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Fórmula lineal:
MgCl2
Número CAS:
Peso molecular:
95.21
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
12352302
MDL number:
Grade:
Molecular Biology
Form:
liquid
Solubility:
water: soluble

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InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

SMILES string

Cl[Mg]Cl

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

grade

Molecular Biology

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

technique(s)

DNA sequencing: suitable

impurities

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

solubility

water: soluble

density

1.080 g/cm3

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for electrophoresis, suitable for molecular biology

application(s)

cell analysis

foreign activity

DNase, RNase, NICKase and protease, none detected

Quality Level

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Este artículo
68475208337449164
form

liquid

form

liquid

form

powder

form

-

solubility

water: soluble

solubility

-

solubility

-

solubility

H2O: slightly soluble(lit.)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

density

1.080 g/cm3

density

-

density

2.32 g/mL at 25 °C (lit.)

density

2.32 g/mL at 25 °C (lit.)

impurities

DNAse, none detected, Protease, none detected, NICKase, none detected, RNAse, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total nitrogen (N)

impurities

<5%

impurities

≤1500.0 ppm Trace Metal Analysis

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation[1]
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.[2]
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.[3]
  • It has been used to protect the liver from oxidative damage.[4]
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.[5]

Features and Benefits

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

General description

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Other Notes

For additional information on our range of Biochemicals, please complete this form.
Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

comparable product

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Protective Effects of Magnesium Chloride on Liver Enzymes and Biomarkers of Oxidative Stress in high fat diet fed Rats
Mohammed et, al
Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria, 34(2), 149-157 (2019)
M J Mc Garrigle et al.
European cells & materials, 31, 323-340 (2016-05-25)
Extracellular matrix (ECM) stiffness and cell density can regulate osteoblast differentiation in two dimensional environments. However, it is not yet known how osteoblast-osteocyte differentiation is regulated within a 3D ECM environment, akin to that existing in vivo. In this study
Yelena Y Grinberg et al.
Journal of neurochemistry, 122(1), 221-229 (2012-04-25)
Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure
Antimo Migliaccio et al.
Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells
Hiroaki Eshima et al.
Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1153-1162 (2020-03-28)
Obesity and aging reduce skeletal muscle contractile function. However, it remains unclear whether obesity additively promotes muscle contractile dysfunction in the setting of aging. In this study, we investigated skeletal muscle contractile function ex vivo and intracellular Ca2+ release in

Protocolos

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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Product Information Sheet

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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