Merck
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M2634

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

Synonym(s):
L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
Enzyme Commission number:
Número MDL:
NACRES:
NA.54

formulario

buffered aqueous glycerol solution

Nivel de calidad

200

specific activity

600-1000 units/mg protein (biuret)

trazas de catión

NH4+: ≤10 μg/mg protein

actividad extraña

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

enviado en

wet ice

temp. de almacenamiento

2-8°C

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Descripción general

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.
Mitochondrial Isozyme

Aplicación

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. [1] It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens. [2]

Acciones bioquímicas o fisiológicas

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C. [3]

Definición de unidad

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Forma física

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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