M2634

Malic Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

• Synonym(s): L-Malate: NAD⁺ oxidoreductase, MDH, Malate Dehydrogenase
• Enzyme Commission number: 1.1.1.37 (BRENDA, IUBMB)
• Número MDL: MFCD00131567
• NACRES: NA.54

Properties

• formulario: buffered aqueous glycerol solution
• Nivel de calidad: 200
• specific activity: 600-1000 units/mg protein (biuret)
• trazas de catión: NH₄⁺: ≤10 μg/mg protein
• actividad extraña: Glutamic-Oxalacetic Transaminase ≤0.01%; Glutamic-Pyruvic Transaminase ≤0.01%
• enviado en: wet ice
• temp. de almacenamiento: 2-8°C

Description

Descripción general

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Mitochondrial Isozyme

Aplicación

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. [1] It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens. [2]

Acciones bioquímicas o fisiológicas

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD

Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C. [3]

Definición de unidad

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Forma física

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)