M4308

Sigma-Aldrich

Monoclonal Anti-MDM2 antibody produced in mouse

clone SMP14, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SMP14, monoclonal

mol wt

antigen ~90 kDa (additional band of approx. 55 kDa)

contains

15 mM sodium azide

species reactivity

human, mouse (weak), rat

application(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(246362)

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General description

Monoclonal Anti-MDM2 (mouse IgG1 isotype) is derived from the SMP14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. The murine double minute (mdm2), an E3 ubiquitin ligase comprises an N-terminal p53 domain. The C-terminal domain encompasses the really interesting new gene (RING) domain, nuclear localization signal (NLS), nucleolar localization signal (NoLS) and a nuclear export signal (NES). MDM2 is mapped to the human chromosome 12q15. It is expressed as different splice variants.

Specificity

Detects the MDM2 protein alone or in complex with E2F1, p53, and retinoblastoma proteins. A slight cross-reactivity is observed with cytokeratins 6, 14, and 16.

Immunogen

synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH.

Application

Monoclonal Anti-MDM2 antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation (1 μg/ml)
  • fluorescence-activated cell sorting (FACS)
  • immunofluorescence (1:200)
  • immunohistochemistry
  • confocal immunofluorescence
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Murine double minute (mdm2) mediates the transactivation of the tumor suppressor p53. MDM2 binds to the histone acetyltransferase p300 transcriptional coactivator/histone acetylase to mediate the degradation of p53. The nuclear localization signal of MDM2 mediates its shuttling from the nucleus to the cytoplasm and mediates p53 degradation. In addition to its relationships with p53, MDM2 activates cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/dimerization partner 1 (DP1). The amplification of the MDM2 gene is detected in the esophagogastric, liver, colon, breast and lung carcinomas. A disturbance in the optimal levels of the MDM2/p53 axis may have an impact on premature aging. Monoclonal antibody reacting specifically with MDM2 is an essential tool in defining the interactions and distributions of MDM2, its function in signaling pathways and mutual regulation of p53.

Physical form

Monoclonal Anti-MDM2 is supplied as ascites fluid containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

Helei Hou et al.
Cancer cell international, 19, 216-216 (2019-08-24)
The MDM2 protein encoded by the mouse double minute 2 (MDM2) gene is the primary negative regulatory factor of the p53 protein. MDM2 can ligate the p53 protein via its E3 ubiquitin ligase, and the ubiquitinated p53 can be transferred...
Tomoo Iwakuma et al.
Molecular cancer research : MCR, 1(14), 993-1000 (2004-01-07)
The murine double minute 2 (mdm2) gene encodes a negative regulator of the p53 tumor suppressor. Amplification of mdm2 or increased expression by unknown mechanisms occurs in many tumors. Thus, increased levels of MDM2 would inactivate the apoptotic and cell...
Y Zhou et al.
Oncogene, 37(8), 1086-1094 (2017-11-07)
Esophageal squamous cell carcinoma (ESCC) is highly prevailing in Asia and it is ranked in the most aggressive squamous cell carcinomas. High-frequency loss of heterozygosity occurred in chromosome 14q11.2 in many tumors including ESCC, suggesting that one or more tumor-suppressor...
Atsunori Oga et al.
Oncology reports, 26(6), 1393-1398 (2011-09-07)
We analyzed 10 adenoid cystic carcinomas (ACCs) of the salivary glands by array-based comparative genomic hybridization (a-CGH) using DNA chips spotted ...
Antoine Italiano et al.
International journal of cancer, 122(10), 2233-2241 (2008-01-25)
Data concerning the fine structure of the 12q13-15 amplicon which contains MDM2 and CDK4 in well-differentiated and dedifferentiated liposarcomas (WDLPS/DDLPS) are scarce. We investigated a series of 38 WDLPS/DDLPS using fluorescence in situ hybridization analysis with 17 probes encompassing the...

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