M4655

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): EMEM, MEM
• NACRES: NA.75

Properties

• Nivel de calidad: 400
• sterility: sterile-filtered
• formulario: liquid
• technique(s): cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• componentes: sodium pyruvate: no; phenol red: yes; Earle’s salts (5% CO₂): yes; HEPES: no; NaHCO₃: yes; L-glutamine: yes
• enviado en: ambient
• temp. de almacenamiento: 2-8°C

Description

Descripción general

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Envase

500, 500, 500 mL

1, 1, 10 L

Aplicación

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Otras notas

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable