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M4943

Sigma-Aldrich

Anti-phospho-MEK4 (pSer257/pThr261) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Número MDL:
NACRES:
NA.44

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

species reactivity

human, frog, mouse

technique(s)

western blot: 0.1-1.0 μg/mL using extracts prepared from GST-tagged fusion protein expressing MEK 4.

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... MAP2K4(6416)
mouse ... Map2k4(26398)

Descripción general

Mitogen-Activated Protein Kinase Kinase 4 (MEK 4 or MKK4) is a 42 kDa member of a tyrosine/threonine protein kinase family that activates the c-Jun NH2-terminal kinases (JNK), which is part of the inflammation/stress signaling pathway. The mutation of the MEK 4 gene in some carcinomas indicates that it may also have a role as a tumor suppressor. Phosphorylation of MEK4 by MEKK1 on serine 257 and threonine 261 in the catalytic domain activates the protein and enables MEK 4 to phosphorylate JNK
This antibody specifically recognizes human MEK 4 double phosphorylated at serine 257 and threonine 261. Mouse and frog MEK 4 (100% homology) may cross react.

Inmunógeno

synthetic MEK4-derived phosphopeptide that is phosphorylated at serine 257 and threonine 261.

Aplicación

Anti-phospho-MEK4 (Tyr397) antibody may be used for detection by immunoblotting at a working concentration of 0.1-1.0 μg/mL.

Forma física

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

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