M5257

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

• Synonym(s): L-Malate:NADP⁺ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme
• CAS Number: 9028-47-1
• Enzyme Commission number: 1.1.1.40 (BRENDA, IUBMB)
• Número de EC: 232-834-8
• Número MDL: MFCD00131568
• NACRES: NA.54

Properties

• origen biológico: chicken liver
• Nivel de calidad: 200
• formulario: ammonium sulfate suspension
• specific activity: 10-30 units/mg protein (modified Warburg-Christian)
• temp. de almacenamiento: 2-8°C

Description

Descripción general

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Aplicación

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Envase

5, 25, 50, 100 units

Acciones bioquímicas o fisiológicas

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

Definición de unidad

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO_2, and NADPH per min at pH 7.4 at 25 °C.

Forma física

Suspension in 2.9 M (NH₄)₂SO₄ solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na₄EDTA, pH 6.0

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable