M5655

Thiazolyl Blue Tetrazolium Bromide

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

• Synonym(s): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
• Empirical Formula (Hill Notation): C₁₈H₁₆BrN₅S
• CAS Number: 298-93-1
• Molecular Weight: 414.32
• Beilstein: 4081397
• Número de EC: 206-069-5
• Número MDL: MFCD00011964
• ID de la sustancia en PubChem: 24897030
• NACRES: NA.75

Properties

• Línea del producto: BioReagent
• Nivel de calidad: 200
• ensayo: ≥97.5% (HPLC)
• formulario: powder
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• mp: 195 °C (dec.) (lit.)
• solubilidad: H₂O: 5 mg/mL
• temp. de almacenamiento: 2-8°C
• SMILES string: [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Description

Aplicación

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.

Envase

15 mg in poly bottle

100, 500 mg in glass insert

1, 1 g

Safety Information

• pictogramas: GHS07,GHS08
• Palabra de señalización: Warning
• Frases de peligro: H315 - H319 - H335 - H341
• Consejos de prudencia: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)