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M8250

Sigma-Aldrich

MES hydrate

≥99.5% (titration)

Sinónimos:
4-Morpholineethanesulfonic acid, 2-(N-Morpholino)ethanesulfonic acid hydrate
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
Número de CAS:
Peso molecular:
195.24 (anhydrous basis)
Número MDL:
eCl@ss:
32129211
ID de la sustancia en PubChem:

Nivel de calidad

400

ensayo

≥99.5% (titration)

formulario

crystalline powder

intervalo de pH útil

5.5 - 6.7

pKa 

6.1

solubilidad

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

temp. de almacenamiento

room temp

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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Descripción general

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Aplicación

MES hydrate has been used in the MS (Murashige and Skoog) media for growth of Arabidopsis seedlings. It has been added in the cytoskeleton buffer during immunofluorescence study.

Envase

25, 50 kg in poly drum
25, 100, 250 g in poly bottle
1 kg in poly bottle

Nota de preparación

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Almacenamiento y estabilidad

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Otras notas

Easily compare specifications for MES products with the MES specification table.
Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. This product is tested at 20g/80mL water, or 1.3 M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65 M.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificado de Análisis

Certificado de origen

Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana.
Bigeard J et al.
Proteomics, 14, 2141-2141 (2014)
Negative effect of heat shock on feline calicivirus release from infected cells is associated with the control of apoptosis.
Alvarez-Sanchez C et al.
Virus Research, 198, 44-44 (2015)
Data for Biochemical Research, Dawson, R.M.C. et al, 410-410 (1987)
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, Carlos MH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)
Good, Norman E. et al.
Biochemistry, 5, 467- 477 (1966)

Protocolos

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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