Merck
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M8403

Sigma-Aldrich

McCoy′s 5A Medium

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
5A basal medium
Número de EC:
Número MDL:
NACRES:
NA.75

Nivel de calidad

400

esterilidad

sterile-filtered

formulario

liquid

technique(s)

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

enviado en

ambient

temp. de almacenamiento

2-8°C

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Descripción general

McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.

Aplicación

McCoy′s 5A Medium has been used to maintain:

  • G-361 melanoma cells;
  • 87-MG cells
  • human colon carcinoma cell line HCT116

Acciones bioquímicas o fisiológicas

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Reconstitución

Supplement with 0.22 g/L L-glutamine.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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