M8787

Magnesium chloride solution

PCR Reagent, 25 mM MgCI₂ solution for PCR

• Sinónimos: PCR optimization
• Fórmula lineal: MgCl₂
• Número CAS: 7786-30-3
• Peso molecular: 95.21
• NACRES: NA.52
• PubChem Substance ID: 57654422
• UNSPSC Code: 12352302
• MDL number: MFCD00011106
• Form: liquid

Propiedades

• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• SMILES string: Cl[Mg]Cl
• InChI key: TWRXJAOTZQYOKJ-UHFFFAOYSA-L
• form: liquid
• packaging: vial of 1.5 mL
• concentration: 25 mM±1 mM
• technique(s): PCR: suitable
• color: colorless
• application(s): agriculture; diagnostic assay manufacturing
• foreign activity: DNase, RNase, none detected
• storage temp.: 2-8°C
• Quality Level: 200, 300

Descripción

Application

Magnesium chloride solution has been used as a component of the:

• amplification mixture for polymerase chain reaction (PCR)[1]
• amplification mixture in an inter simple sequence repeats (ISSR)-PCR[2]
• reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)[3]
• PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA[4]
• reaction mix for RT-PCR to assess the gene expression[5]

Suitable for optimization of polymerase chain reactions.

General description

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)