M8823
200
magnetic beads
affinity isolated antibody
primary antibodies
M2, monoclonal
suspension
2 yr at -20 °C
proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
20-75 μm
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
IgG1
≥0.6 mg/mL binding capacity
wet ice
−20°C
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12 - Non Combustible Liquids
WGK 1
Not applicable
Not applicable
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Técnicas, reactivos y protocolos de purificación de proteínas para purificar proteínas recombinantes utilizando métodos como la cromatografía de intercambio iónico, de exclusión por tamaño y de afinidad.
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Tecnologías de expresión proteica para expresar proteínas recombinantes en sistemas de expresión de E. coli, insectos, levaduras y mamíferos destinadas a la investigación fundamental y al apoyo a la producción de tratamientos y vacunas.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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