M8823
Anti-FLAG® M2 Magnetic Beads
affinity isolated antibody
Nivel de calidad
200
conjugado
magnetic beads
forma del anticuerpo
affinity isolated antibody
antibody product type
primary antibodies
clon
M2, monoclonal
formulario
suspension
caducidad
2 yr at -20 °C
analyte chemical class(es)
proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
tamaño microesfera
20-75 μm
matriz
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
isotipo
IgG1
capacidad
≥0.6 mg/mL binding capacity
enviado en
wet ice
temp. de almacenamiento
−20°C
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Related Categories
Descripción general
Aplicación
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
Browse additional application references in our FLAG® Literature portal.
Envase
Características y beneficios
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest
Forma física
Otras notas
Información legal
Código de clase de almacenamiento
12 - Non Combustible Liquids
WGK
WGK 1
Punto de inflamabilidad F
Not applicable
Punto de inflamabilidad C
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
Enter Lot Number to search for Certificate of Origin (COO).
Articles
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
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Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
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Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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