Merck
All Photos(3)

M8823

Millipore

Anti-FLAG® M2 Magnetic Beads

affinity isolated antibody

Synonym(s):
Anti-ddddk, Anti-dykddddk, Flag Affinity resin, Monoclonal ANTI-FLAG M2 antibody produced in mouse, FLAG magnetic affinity resin, FLAG resin for high throughput

Nivel de calidad

200

conjugado

magnetic beads

forma del anticuerpo

affinity isolated antibody

antibody product type

primary antibodies

clon

M2, monoclonal

formulario

suspension

caducidad

2 yr at -20 °C

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

tamaño microesfera

20-75 μm

matriz

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

isotipo

IgG1

capacidad

≥0.6 mg/mL binding capacity

enviado en

wet ice

temp. de almacenamiento

−20°C

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Descripción general

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

Aplicación

Suitable for immunoprecipitation procedures.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Browse additional application references in our FLAG® Literature portal.

Envase

1 mL in poly bottle

Características y beneficios

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

Forma física

Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).

Otras notas

Do not use magnetic stirring system as this will destroy the resin beads.

Información legal

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

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