M9004

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

• Synonym(s): L-Malate:NAD⁺ oxidoreductase, MDH
• Enzyme Commission number: 1.1.1.37 (BRENDA, IUBMB)
• Número de EC: 232-622-5
• Número MDL: MFCD00131567
• NACRES: NA.54

Properties

• origen biológico: bovine heart
• Nivel de calidad: 200
• formulario: ammonium sulfate suspension
• specific activity: 2000-4000 units/mg protein (modified Warburg-Christian)
• mol peso: 35 kDa
• impurezas: ≤0.01% Glutamic-Oxalacetic Transaminase; ≤0.01% Glutamic-Pyruvic Transaminase
• temp. de almacenamiento: 2-8°C

Description

Descripción general

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Aplicación

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Envase

1000, 2500, 5000, 10000, 25000 units

Definición de unidad

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Forma física

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)