M9309

McCoy′s 5A Medium

Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): 5A basal medium
• Número MDL: MFCD00217560

Properties

• Nivel de calidad: 400
• sterility: sterile-filtered
• formulario: liquid
• technique(s): cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• componentes: NaHCO₃: 2.2 g/L; L-glutamine: 0.21 g/L; phenol red: 0.011 g/L; glucose: 3.0 g/L (Dextro)
• enviado en: ambient
• temp. de almacenamiento: 2-8°C

Description

Descripción general

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Aplicación

McCoy′s 5A Medium has been used:

• to culture HCT-116?cells
• to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
• to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

Envase

500, 500 mL

Acciones bioquímicas o fisiológicas

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable