Merck

M9942

Sigma-Aldrich

Anti-MAP2 antibody, Mouse Monoclonal

clone HM-2, purified from hybridoma cell culture

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Sinónimos:
Monoclonal Anti-MAP2 antibody produced in mouse, MAP2 Antibody - Anti-MAP2 antibody, Mouse Monoclonal, Map2 Antibody
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

HM-2, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~280 kDa

species reactivity

rat, chicken, human, mouse, bovine, quail

envase

antibody small pack of 25 μL

concentración

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using rat brain extract

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

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Este artículo
M2320M4403M1406
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

clone

HM-2, monoclonal

clone

AP-20, monoclonal

clone

HM-2, monoclonal

clone

AP-20, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

-

mol wt

antigen ~280 kDa

mol wt

apparent mol wt 280 kDa

mol wt

antigen ~280 kDa

mol wt

apparent mol wt 280 kDa

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Descripción general

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology.
MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.

Especificidad

Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.
This antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

Inmunógeno

rat brain microtubule-associated proteins (MAPs).

Aplicación

Anti-MAP2 antibody has been used in immunofluorescence. It has also been used in immunohistochemistry.
May be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells.
By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract.

Acciones bioquímicas o fisiológicas

MAP2 (microtubule-associated protein 2) expression is monitored to predict the disease progression in glioma. MAP2 is critical in the formation of dendritic protrusions in astrocytes and oligodendrocytes. In eukaryotes, MAP2 is essential for spindle assembly and chromosome segregation.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 C for up to one month. For extended storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Remarkable reduction of MAP2 in the brains of scrapie-infected rodents and human prion disease possibly correlated with the increase of calpain.
Guo Y, et al.
PLoS ONE, 7, e30163-e30163 (2012)
The MAP2/Tau family of microtubule-associated proteins.
Dehmelt L and Halpain S
Genome Biology, 6(1), 204-204 (2005)
Expression of EAAT2 in neurons and protoplasmic astrocytes during human cortical development
DeSilva T M, et al.
The Journal of Comparative Neurology, 520(17), 3912-3932 (2012)
Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair
Li X, et al.
Biomaterials, 34(21), 5107-5116 (2013)
miR-484/MAP2/c-Myc-positive regulatory loop in glioma promotes tumor-initiating properties through ERK1/2 signaling.
Yi R, et al.
Journal of Molecular Histology, 49(2) (2018)

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