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MIDI67
PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
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Synonym(s):
Green PKH membrane midi labeling kit
NACRES:
NA.32
envase
pkg of 1 kit
Nivel de calidad
manufacturer/tradename
Distributed for Phanos Technologies
condiciones de almacenamiento
protect from light
technique(s)
flow cytometry: suitable
fluorescencia
λex 490 nm; λem 502 nm (PKH67 dye)
application(s)
cell analysis
detection
detection method
fluorometric
enviado en
ambient
temp. de almacenamiento
room temp
Related Categories
Descripción general
This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Aplicación
PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
- labeling exosomes
- extracellular vesicles (EVs)l
- normal and sickle red blood cells (RBCs)
- Raji cells
Envase
1 kit
Ligadura / enlace
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Información legal
CellVue is a registered trademark of Phanos Technologies
Kit Components Only
Product No.
Description
- PKH67 Dye 2 x 0.1
- Diluent C 6 x 10
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Flam. Liq. 2
Código de clase de almacenamiento
3 - Flammable liquids
WGK
WGK 1
Punto de inflamabilidad F
57.2 °F - closed cup
Punto de inflamabilidad C
14 °C - closed cup
Certificate of Analysis
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Certificate of Origin
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Product Information Sheet
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