MIDI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

NACRES:
NA.32

packaging

pkg of 1 kit

Quality Level

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

application(s)

cell analysis: suitable
detection: suitable
flow cytometry: suitable

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Categorías relacionadas

General description

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Application

PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
  • labeling exosomes  
  • extracellular vesicles (EVs)l
  • normal and sickle red blood cells (RBCs)
  • Raji cells

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Solo componentes del kit

Referencia del producto
Descripción

  • PKH67 Dye 2 x 0.1

  • Diluent C 6 x 10

pictograms

FlameExclamation mark

signalword

Danger

RIDADR

UN 3316 9

WGK Germany

WGK 1

Flash Point F

57.2 °F - closed cup

Flash Point C

14 °C - closed cup

Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The...
Ying Liu et al.
Kidney diseases (Basel, Switzerland), 6(6), 422-433 (2020-12-15)
Levels of urinary microvesicles, which are increased during various kidney injuries, have diagnostic potential for renal diseases. However, the significance of urinary microvesicles as a renal disease indicator is dampened by the difficulty to ascertain their cell source. The aim...
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The...
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
Artículos
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
Más información
A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.
Más información
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
Más información

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