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N3755

Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym(s):
Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
NACRES:
NA.54

origen biológico

Staphylococcus aureus

Nivel de calidad

300

formulario

lyophilized powder

specific activity

100-300 units/mg protein

composición

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

application(s)

diagnostic assay manufacturing

temp. de almacenamiento

−20°C

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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Aplicación

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Envase

50, 200, 500 units in serum bottle

Acciones bioquímicas o fisiológicas

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.

Definición de unidad

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Otras notas

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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