N5264

Sigma-Aldrich

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

clone NN18, ascites fluid

Sinónimos:
Monoclonal Anti-Neurofilament medium chain
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NN18, monoclonal

mol wt

antigen 160 kDa

contains

15 mM sodium azide

species reactivity

pig, human

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections
immunohistochemistry (frozen sections): suitable
western blot: suitable

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... NEFM(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

General description

Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons.
Monoclonal Anti-Neurofilament 160 (mouse IgG1 iso-type) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins.

Specificity

Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins.

Immunogen

neurofilaments from pig spinal cord.

Application

Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation.
Monoclonal anti-neurofilament 160 antibody has been used in
  • Immunoblotting.
  • Immunohistochemistry
  • Immunofluorescence

Biochem/physiol Actions

Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

F Maina et al.
Genes & development, 11(24), 3341-3350 (1998-02-07)
The development of the nervous system is a dynamic process during which factors act in an instructive fashion to direct the differentiation and survival of neurons, and to induce axonal outgrowth, guidance to, and terminal branching within the target tissue....
Svetlana Gorokhova et al.
PLoS genetics, 10(2), e1004081-e1004081 (2014-02-12)
Neurotrophins and their receptors control a number of cellular processes, such as survival, gene expression and axonal growth, by activating multiple signalling pathways in peripheral neurons. Whether each of these pathways controls a distinct developmental process remains unknown. Here we...
M K Lee et al.
Current opinion in cell biology, 6(1), 34-40 (1994-02-01)
Neurofilaments make up the major intermediate filament system in mature neurons. Recent studies demonstrate that neurofilaments in vivo are obligate heteropolymers and are required for proper radial growth of axons. Furthermore, forced over-expression of neurofilament subunits in transgenic mice shows...
Chihiro Takasaki et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(19), 4995-5006 (2008-05-09)
Glutamate transporters are involved in neural differentiation, neuronal survival, and synaptic transmission. In the present study, we examined glutamate transporter 1 (GLT1) expression in the neonatal somatosensory cortex of C57BL/6 mice, and pursued its role in somatosensory development by comparing...
E Delmas et al.
Cell death & disease, 7, e2079-e2079 (2016-02-06)
Fibroblast growth factor 1 (FGF1) is a prototypic member of the FGFs family overexpressed in various tumors. Contrarily to most FGFs, FGF1 lacks a secretion peptide signal and acts mainly in an intracellular and nuclear manner. Intracellular FGF1 induces cell...

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