N5389

Sigma-Aldrich

Monoclonal Anti-Neurofilament 200 antibody produced in mouse

enhanced validation

clone NE14, ascites fluid

Sinónimos:
Nf200 Antibody, Neurofilament Antibody, Monoclonal Anti-Neurofilament heavy chain, NF200 Antibody - Monoclonal Anti-Neurofilament 200 antibody produced in mouse
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NE14, monoclonal

mol wt

antigen apparent mol wt 200 kDa

contains

15 mM sodium azide as preservative

species reactivity

chicken, human, bovine, guinea pig, rabbit, pig, rat, mouse

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
immunohistochemistry (frozen sections): suitable
microarray: suitable
western blot: suitable

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NEFH(4744)

Categorías relacionadas

General description

Monoclonal Anti-Neurofilament 200 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The neurofilaments are one of the five major groups of intermediate filaments (IFs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD and are named as NEFL (light), NEFM (medium) and NEFH (heavy) respectively. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals.

Immunogen

pig spinal cord.

Application

Monoclonal Anti-Neurofilament 200 antibody produced in mouse has been used in
  • immunohistochemistry
  • immunolabeling
  • immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Neurofilament heavy chain (NEFH) gene was recently identified to cause autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc). This gene plays a role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

Cell cycle kinetics and immunohistochemical characterization of dissociated fetal neocortical cultures: evidence that differentiated neurons have mitotic capacity
Jacobs JS and Miller MW
Dev. Brain Res., 122(1), 67-80 (2000)
Neurofilament proteins are synthesized in nerve endings from squid brain
Crispino M, et al.
Journal of Neurochemistry, 61(3), 1144-1146 (1993)
C Lois et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(5), 2074-2077 (1993-03-01)
Subventricular zone (SVZ) cells proliferate spontaneously in vivo in the telencephalon of adult mammals. Several studies suggest that SVZ cells do not differentiate after mitosis into neurons or glia but die. In the present work, we show that SVZ cells...
Lilla Reiniger et al.
Acta neuropathologica communications, 1, 8-8 (2013-11-21)
Sporadic, inherited and acquired prion diseases show distinct histological patterns of abnormal prion protein (PrP) deposits. Many of the inherited prion diseases show striking histological patterns, which often associate with specific mutations. Most reports have focused on the pattern of...
Jon O Cleary et al.
NeuroImage, 56(3), 974-983 (2011-02-12)
Extensive worldwide efforts are underway to produce knockout mice for each of the ~25,000 mouse genes, which may give new insights into the underlying pathophysiology of neurological disease. Microscopic magnetic resonance imaging (μMRI) is a key method for non-invasive morphological...

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