All Photos(1)
NA9604
GenElute™ HP 96-Well Plasmid Miniprep Kit
sufficient for 4 96-well plate purifications
All Photos(1)
Synonym(s):
plasmid purification kit, 96 well plasmid extraction kit, plasmid isolation kit, high throughput miniprep kit, plasmid extraction kit, GenElute HP Plasmid Kit, Gen Elute, High Throughput Purification, Plasmid Purification, 96 well plasmid isolation kit, 96 well plasmid purification kit, high throughput plasmid kit
NACRES:
NA.52
Nivel de calidad
200
uso
sufficient for 4 96-well plate purifications
temp. de almacenamiento
15-25°C
Related Categories
Descripción general
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.
The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.
The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.
Aplicación
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
Características y beneficios
- Up to 10 μg of high-copy plasmid DNA per well
- Flexibility of both manual and automated formats
- Optimized for production of virus titers from MISSION shRNA plasmid vectors
- No phenol/chloroform extraction or alcohol precipitation required
Información legal
GenElute is a trademark of Sigma-Aldrich Co. LLC
Palabra de señalización
Danger
Frases de peligro
Clasificaciones de peligro
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Órganos de actuación
Central nervous system
Código de clase de almacenamiento
3 - Flammable liquids
WGK
WGK 2
Punto de inflamabilidad F
77.0 °F
Punto de inflamabilidad C
25 °C
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
Enter Lot Number to search for Certificate of Origin (COO).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service