Merck

P0082

Sigma-Aldrich

Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse

~1.0 mg/mL, clone PF-29, purified immunoglobulin, buffered aqueous solution

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Sinónimos:
Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-POU domain, class 5, transcription factor 1

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PF-29, monoclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using NT2/D1 total cell extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 4

Este artículo
P0056O8389SAB4200481
clone

PF-29, monoclonal

clone

polyclonal

clone

polyclonal

clone

SAT-5, monoclonal

Gene Information

human ... POU5F1(5460)

Gene Information

human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

Gene Information

human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

Gene Information

human ... SATB1(6304)

species reactivity

human

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

human, mouse, monkey, rat

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Monoclonal Anti-POU5F1 (Oct4) (mouse IgG2a isotype) is derived from the hybridoma PF-29 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a peptide.
POU5F1 (POU class 5 homeobox 1) gene codes for a transcription factor that binds to octamer sequence. POU5F1 is mapped to human chromosome 6p21.33.

Specificity

Monoclonal Anti-POU5F1 (Oct4) recognizes human POU5F1/Oct4.

Immunogen

amino acids 20-37 of human POU5F1 peptide.

Application

Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse may be used in:
  • immunoblotting
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

POU5F1 (POU class 5 homeobox 1) controls homeostasis of embryonic cell development and pluripotency. The POU5F1 gene is highly expressed in cancer cells and offers resistance against chemotherapy.
POU5F1 protein functions as an early developmental control gene. This protein has been recognized as fundamental in the maintenance of pluripotency of embryonic stem cells and primordial germ cells. POU5F1 protein has been proposed as a useful marker for germ cell tumors (GCTs) that can exhibit pluripotential, specifically seminoma/ dysgerminoma/germinoma and embryonal carcinoma (EC). POU5F1 has also been established as a marker for extragonadal germ cell tumors (GCT) and metastatic tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Optional

Related product

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Para su comodidad, los documentos relacionados con los productos que usted ha comprado con anterioridad se han recopilado en la Biblioteca de documentos.

Visite la Librería de documentos

¿Le resulta difícil encontrar su producto o lote/número de lote?

Las referencias de los producto se combinan con los tamaños de envase y las cantidades cuando se muestran en la página web, por ejemplo: T1503-25G. Asegúrese de introducir el número de producto ÚNICAMENTE en el campo del Número de producto (ejemplo: T1503).

Ejemplo

T1503
Referencia del producto
-
25G
Tamaño del envase/cantidad

Otros ejemplos

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

introducir como 1.000309185)

¿Tiene algún problema? No dude en ponerse en contacto con nosotros Servicio técnico para asistencia

Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch».

Productos Aldrich

  • Si encuentra un número de lote como TO09019TO, introduzca el número de lote 09019TO sin las dos primeras letras.

  • Si encuentra un número de lote con un código de entrada como 05427ES-021, introduzca el número 05427ES sin el código de entrada -021.

  • Si encuentra un número de lote con un código de entrada como STBB0728K9, introduzca el código de lote STBB0728 sin el código de entrada K9.

¿No ha encontrado lo que estaba buscando?

En algunos casos, puede que un COA no esté disponible en la página web. Si su búsqueda no encontró el COA puede solicitarlo.

Solicitar COA

Timothy D Jones et al.
The American journal of surgical pathology, 28(7), 935-940 (2004-06-30)
OCT4 (POU5F1) is a transcription factor expressed in embryonic stem and germ cells and is involved in the regulation and maintenance of pluripotency. It has been detected in primary testicular germ cell tumors with pluripotent potential, seminoma, and embryonal carcinoma.
Ming-Tse Sung et al.
Human pathology, 37(6), 662-667 (2006-05-31)
Correctly diagnosing a metastatic germ cell tumor after chemotherapy may be challenging because of the diverse morphological manifestations of postchemotherapy tumors. Both OCT4 and CD30 are sensitive markers for the identification of primary embryonal carcinomas; however, loss of expression of
Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer.
Lu C S, et al.
Oncotarget, 8(19), 30844-30844 (2017)
Clinical significance of putative markers of cancer stem cells in gastric cancer: A retrospective cohort study.
Chen X L, et al.
Oncotarget, 7(38), 62049-62049 (2016)
Minimum regions of genomic imbalance in stage I testicular embryonal carcinoma and association of 22q loss with relapse.
Gilbert D C, et al.
Genes Chromosomes Cancer, 50(3), 186-195 (2011)

Artículos

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico