P0781

Sigma-Aldrich

Penicillin-Streptomycin

with 10,000 units penicillin and 10 mg streptomycin per mL in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture

Sinónimos:
Pen-Strep
PubChem Substance ID:
NACRES:
NA.76

Quality Level

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

concentration

100 ×

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

Mode of action

cell wall synthesis | interferes
protein synthesis | interferes

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

shipped in

dry ice

storage temp.

−20°C

SMILES string

CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(O)=O.CNC[C@H]4[C@H](O)[C@@H](O)[C@H](CCO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)C5(O)C=O)O[C@H]6[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]6NC(N)=N

InChI

1S/C23H43N7O12.C16H18N2O4S/c1-7-23(38,6-32)18(42-19-8(5-28-2)12(33)13(34)9(40-19)3-4-31)20(39-7)41-17-11(30-22(26)27)14(35)10(29-21(24)25)15(36)16(17)37;1-16(2)12(15(21)22)18-13(20)11(14(18)23-16)17-10(19)8-9-6-4-3-5-7-9/h6-20,28,31,33-38H,3-5H2,1-2H3,(H4,24,25,29)(H4,26,27,30);3-7,11-12,14H,8H2,1-2H3,(H,17,19)(H,21,22)/t7-,8-,9-,10+,11-,12-,13-,14+,15-,16+,17+,18-,19-,20-,23+;11-,12+,14-/m01/s1

InChI key

KMOOCZWLFBSQCW-XFOLRDBHSA-N

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General description

Penicillin streptomycin, also known as Pen-Strep, is the most commonly used antibiotic solution for culture of mammalian cells. Penicillin Streptomycin solution is used to maintain sterile conditions during cell culture, and they show few adverse effects on the cultured cells.

Application

Penicillin-Streptomycin solution is used to supplement cell culture media to control bacterial contamination. It has also been used for the preparation of lipid emulsions. It is recommended for use in cell culture applications at 10 mL/L.

Components

This product is formulated to contain 10,000 units penicillin and 10 mg streptomycin/mL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.
20x100ml,20ml,100ml

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

D T Stein et al.
The Journal of clinical investigation, 100(2), 398-403 (1997-07-15)
Lowering of the elevated plasma FFA concentration in 18- 24-h fasted rats with nicotinic acid (NA) caused complete ablation of subsequent glucose-stimulated insulin secretion (GSIS). Although the effect of NA was reversed when the fasting level of total FFA was...
Bryan Brown et al.
Tissue engineering, 12(3), 519-526 (2006-04-04)
The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The basement membrane is a specialized form of the ECM that supports and facilitates...
184: Toxic effects of antibiotics routinely used in cell culture on primary cultures of dissociated mouse brain cells.
Amonn F
Pediatric Research, 10(10), 900-900 (1976)
J H Martínez-Liarte et al.
Pigment cell research, 8(2), 83-88 (1995-04-01)
Penicillin and streptomycin, the most widely used antibiotics in mammalian cell cultures, caused a moderate stimulation in dopa oxidase and tyrosine hydroxylase activities, but a slight inactivation in the dopachrome tautomerase activity of B16/F10 melanoma cells at the routine concentration...
R M Pereira et al.
Animal reproduction science, 98(3-4), 293-301 (2006-04-29)
An excessive lipid content in embryo cells is a consequence of embryo culture in the presence of serum which is suggested to be responsible for their high susceptibility to cryopreservation. The objective of the present study was to examine the...

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