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o-Phenylenediamine dihydrochloride

peroxidase substrate

Fórmula lineal:
Número de CAS:
Peso molecular:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


white to off-white


258 °C (dec.) (lit.)

storage temp.


SMILES string




InChI key


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o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.


10, 25, 50, 100 g in glass bottle
1 kg in glass bottle


May darken in storage.



Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

Areano Ethério Moreira de Farias et al.
Tropical animal health and production, 51(4), 745-752 (2018-11-14)
Goat breeding in the Northeast region of Brazil plays an important socioeconomic role. However, there are significant losses caused by sanitary deficits and infectious diseases, particularly caseous lymphadenitis (CL). Although CL is considered endemic in Northeastern Brazil, a comprehensive and...
Bikash R Sahu et al.
The Journal of infectious diseases, 198(3), 434-443 (2008-07-01)
Filaria-specific antibodies of immunoglobulin (Ig) G, IgE, and IgM isotypes have been correlated with acquired immunity in the literature, but the status of filaria-specific IgA and its role in human filariasis has not been addressed. The present study attempts to...
Andrew K I Falconar et al.
Clinical and vaccine immunology : CVI, 13(9), 1044-1051 (2006-09-09)
We compared dengue virus (DV) isolation rates and tested whether acute primary (P) and acute/probable acute secondary (S/PS) DV infections could be correctly classified serologically when the patients' first serum (S1) samples were obtained 1 to 3 days after the...
E O'Connor et al.
Journal of immunological methods, 229(1-2), 155-160 (1999-11-11)
The ability to detect a protein is always limited to the sensitivity of the assays available. Progress in improving the sensitivity of protein detection will allow a more complete understanding of biological systems. Of particular interest to the field of...
Guy Mollett et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 69(7), 1130-1135 (2018-12-13)
Visceral leishmaniasis (VL), caused by the Leishmania donovani complex, is a fatal, neglected tropical disease that is targeted for elimination in India, Nepal, and Bangladesh. Improved diagnostic tests are required for early case detection and for monitoring the outcomes of...


Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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