P2922

Endoproteinase Glu-C from Staphylococcus aureus V8

Type XVII-B, lyophilized powder, 500-1,000 units/mg solid

• Synonym(s): V8 Protease
• CAS Number: 66676-43-5
• Enzyme Commission number: 3.4.21.19 (BRENDA, IUBMB)
• Número MDL: MFCD01324998
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• tpo: Type XVII-B
• Nivel de calidad: 200
• formulario: lyophilized powder
• specific activity: 500-1,000 units/mg solid
• mol peso: 29 kDa
• purificado por: chromatography
• enviado en: wet ice
• temp. de almacenamiento: −20°C
• Gene Information: Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)

Description

Aplicación

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments.

It is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.

Envase

100, 500, 2500 units

Acciones bioquímicas o fisiológicas

Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

Definición de unidad

One unit will hydrolyze 1 μmole of N-t-Boc-L-glutamic acid α-phenyl ester per min at pH 7.8 at 37 °C. One unit is equivalent to ~0.004 casein digestion unit.

Safety Information

• pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317 - H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves