Merck
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P3910

Sigma-Aldrich

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
eCl@ss:
32160410
NACRES:
NA.84

formulario

lyophilized powder

Nivel de calidad

100

calidad

for use in Total Dietary Fiber Assay, TDF-100A

mol peso

27 kDa

application(s)

food and beverages

enviado en

wet ice

temp. de almacenamiento

2-8°C

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Especificidad

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Aplicación

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Envase

500 mg in poly bottle

Acciones bioquímicas o fisiológicas

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Propiedades físicas

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Definición de unidad

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 2

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Technical Information

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