P3910

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

• Synonym(s): Proteinase from Bacillus licheniformis, Subtilisin A
• CAS Number: 9014-01-1
• Enzyme Commission number: 3.4.21.62 (BRENDA, IUBMB)
• Número de EC: 232-752-2
• Número MDL: MFCD00132092
• eCl@ss: 32160410
• NACRES: NA.84

Properties

• formulario: lyophilized powder
• Nivel de calidad: 100
• calidad: for use in Total Dietary Fiber Assay, TDF-100A
• mol peso: 27 kDa
• application(s): food and beverages
• enviado en: wet ice
• temp. de almacenamiento: 2-8°C

Description

Especificidad

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Aplicación

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Envase

500 mg in poly bottle

Acciones bioquímicas o fisiológicas

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Propiedades físicas

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Definición de unidad

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

Safety Information

• pictogramas: GHS05,GHS07,GHS08,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H302 - H315 - H318 - H334 - H335 - H410
• Consejos de prudencia: P273 - P280 - P301 + P312 + P330 - P302 + P352 - P305 + P351 + P338 + P310
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 2
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves